{"id":42558,"date":"2023-10-11T11:46:25","date_gmt":"2023-10-11T11:46:25","guid":{"rendered":"https:\/\/www.hgi-cgs.hr\/?page_id=42558"},"modified":"2023-10-11T11:58:47","modified_gmt":"2023-10-11T11:58:47","slug":"asistentica-ivona-banicek-boravila-na-globe-institutu-sveucilista-u-kopenhagenu-u-sklopu-erasmus-strucne-prakse","status":"publish","type":"page","link":"https:\/\/www.hgi-cgs.hr\/en\/asistentica-ivona-banicek-boravila-na-globe-institutu-sveucilista-u-kopenhagenu-u-sklopu-erasmus-strucne-prakse\/","title":{"rendered":"Asistentica Ivona Bani\u010dek boravila na Globe institutu Sveu\u010dili\u0161ta u Kopenhagenu u sklopu Erasmus+ stru\u010dne prakse"},"content":{"rendered":"

[et_pb_section fb_built=”1″ _builder_version=”4.19.1″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][\/et_pb_section][et_pb_section fb_built=”1″ fullwidth=”on” _builder_version=”4.16″ custom_padding=”0px||0px|||” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_fullwidth_slider _builder_version=”4.16″ min_height=”112px” height=”400px” custom_margin=”0px||||false|false” custom_padding=”0px||18px||false|false” auto=”on” auto_ignore_hover=”on” locked=”off” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/Br\u010di\u0107_Drvenik02-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/ban2-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/20190228_094232-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/ban3-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/Bijele_stijene-Gorski_kotar-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/ban4-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][et_pb_slide heading=”Hrvatski geolo\u0161ki institut” _builder_version=”4.16″ header_font=”|||on|||||” background_image=”https:\/\/www.hgi-cgs.hr\/wp-content\/uploads\/2020\/03\/ban5-scaled.jpg” background_enable_image=”on” locked=”off” global_colors_info=”{}” sticky_transition=”on” theme_builder_area=”post_content”][\/et_pb_slide][\/et_pb_fullwidth_slider][\/et_pb_section][et_pb_section fb_built=”1″ _builder_version=”4.16″ custom_padding=”||0px|||” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_row _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”4_4″ _builder_version=”4.16″ global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.20.4″ text_font_size=”32px” header_2_text_color=”#1e5096″ global_colors_info=”{}” theme_builder_area=”post_content”]<\/p>\n

NOVOSTI
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Asistentica Ivona Bani\u010dek boravila na Globe institutu Sveu\u010dili\u0161ta u Kopenhagenu u sklopu Erasmus+ stru\u010dne prakse<\/h2>\n

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Od svibnja do srpnja 2023. godine asistentica Hrvatskog geolo\u0161kog instituta Ivona Bani\u010dek boravila je preko Erasmus+ stru\u010dne prakse na danskom istra\u017eiva\u010dkom institutu za paleogenetiku \u2013 Globe institut Sveu\u010dili\u0161ta u Kopenhagenu gdje je izu\u010davala metodu drevne DNK kod doc.dr.sc. Ane Prohaske.<\/p>\n

Drevna DNK (eng. ancient DNA \u2013 aDNA<\/em>) predstavlja preostalu degradiraju\u0107u geneti\u010dku materiju uginulih organizama koja je jo\u0161 uvijek iskoristiva za rekonstrukciju i determinaciju vrsti. Paralelno istra\u017eivanju aDNK, razvijala su se i istra\u017eivanja tragova organizama u okoli\u0161u, odnosno okoli\u0161na DNK (eng. environmental DNA \u2013 eDNA<\/em>) gdje se analizira totalni sadr\u017eaj genetskog materijala u uzorku nakon \u010dega se determiniraju pojedine vrste. Kao zasebna cjelina, izdvaja se i sedimentna DNK (eng. sedimentary ancient DNA \u2013 sedaDNA<\/em>) koja je fokusirana isklju\u010divo na nalaze DNK u sedimentima.<\/p>\n

\u0160irok je spektar primjenjivosti metoda: od biologije, taksonomije, monitoringa bioraznolikosti i kvantifikacije utjecaja recentnih klimatskih promjena na organizme; preko arheologije, determinacije razvoja \u010dovjeka, migracija ljudi, povijesnog pregleda bolesti; do paleoekologije, rekonstrukcije pro\u0161lih klimatskih promjena, prilagodbe vrsta, izumiranja, antropolo\u0161kog utjecaja na okoli\u0161. Metode nude mogu\u0107nost povezivanja flore i faune kroz vrijeme i prostor na \u0161irokom podru\u010dju te upotpunjuje peludne zapise koji su uvjetovani brzinom donosa, dalekose\u017enim raspr\u0161enjem i vegetativnim rastom biljaka.<\/p>\n

Osnovni ograni\u010davaju\u0107i faktori metoda su post-mortem degradacija DNK molekule kojom se skra\u0107uje na sve manje fragmente tijekom vremena, i problem kontaminacije. Izvor kontaminacije mo\u017ee biti od uzorka (nepa\u017eljivo uzorkovanje ili skladi\u0161tenje) i unutar laboratorija (pogre\u0161kom laboratorijskog osoblja). Stoga se velika pa\u017enja posve\u0107uje spre\u010davanju kontaminacije redovitim \u010di\u0161\u0107enjem, UV sterilizacijom, no\u0161enjem za\u0161titnih odjela i opreme, zabrani uno\u0161enja mobitela, utiskivanjem \u010distog zraka te adekvatnom cirkulacijom.<\/p>\n

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Slika 1. Primjer \u010distog laboratorija (preuzeto s https:\/\/www.germfree.com\/applications\/cell-gene-therapy-cleanrooms\/)<\/em><\/em><\/p>\n

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Istra\u017eivanja drevne okoli\u0161ne sedaDNK kojom se bavi istra\u017eiva\u010dka grupa doc.dr.sc. Ane Prohaske, mogu se razlo\u017eiti na nekoliko etapa: uzorkovanje sedimentnih jezgri u sterilnim uvjetima, smrzavanje uzoraka, njihov transport i priprema u \u010distom laboratoriju Globe instituta, ekstrakcije DNK molekula odre\u0111enim protokolom, provjera du\u017eina lanaca i kvalitete DNK na BioAnalyzer instrumentu te u slu\u010daju povoljnih rezultata, uzorci se \u0161alju na sekvenciranje, odnosno automatizirano odre\u0111ivanje slijeda nukleotida u molekuli te usporedbu detektiranih DNK molekula s bazama podataka i pridru\u017eivanje pojedinim vrstama.<\/p>\n

Asistentica je istra\u017eivanje dovela do koraka provjere kvalitete i du\u017eine DNK molekula te preostaje nastaviti rad s bioinformatikom \u0161to je pauzirano do osiguravanja sredstava za ponovni dolazak. Rezultati rada \u0107e se koristiti u Ivoninoj doktorskoj disertaciji \u0161to direktno doprinosi kvartarnim istra\u017eivanjima u Hrvatskoj.<\/p>\n

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Slika 2. Asistentica analizira kvalitetu i du\u017einu ekstrahiranih DNK molekula na BioAnalyzeru<\/em><\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":"

NOVOSTIAsistentica Ivona Bani\u010dek boravila na Globe institutu Sveu\u010dili\u0161ta u Kopenhagenu u sklopu Erasmus+ stru\u010dne prakseOd svibnja do srpnja 2023. godine asistentica Hrvatskog geolo\u0161kog instituta Ivona Bani\u010dek boravila je preko Erasmus+ stru\u010dne prakse na danskom istra\u017eiva\u010dkom institutu za paleogenetiku \u2013 Globe institut Sveu\u010dili\u0161ta u Kopenhagenu gdje je izu\u010davala metodu drevne DNK kod doc.dr.sc. Ane Prohaske. Drevna […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"class_list":["post-42558","page","type-page","status-publish","hentry"],"aioseo_notices":[],"aioseo_head":"\n\t\t\n\t\n\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t\n\t\t